?一、物種組成分析內(nèi)容及意義
a)物種相對(duì)豐度圖
根據(jù) OTU 注釋結(jié)果分別在各個(gè)分類水平：domain（域），kingdom（界），phylum（門），class（綱），order（目），family（科），genus（屬），species（種）統(tǒng)計(jì)各樣品的物種相對(duì)豐度并作圖（依據(jù)給定的物種比例分布表格）。
含義：樣品中包含何種物種；物種豐度是多少；在不同樣品間是否存在某些物種的變化及變化趨勢(shì)。
b)優(yōu)勢(shì)物種（關(guān)鍵物種） Heatmap 圖
它可以直觀地將數(shù)據(jù)值的大小以定義的顏色深淺表示出來。常需要將數(shù)據(jù)進(jìn)行物種或樣品間豐度相似性聚類。
目的及意義：可將高豐度和低豐度的物種分塊聚集，通過顏色梯度及相似程度來反映多個(gè)樣品在各分類水平上群落組成的相似性和差異性。
c)OTU 分布 Venn 圖
多個(gè)樣品中所共有和獨(dú)有的 OTU展示，依據(jù)是共有和獨(dú)有OTU表格。
意義：體現(xiàn)共性和獨(dú)特性上的差異。
二、物種組成分析在科技論文中的描述
a）物種相對(duì)豐度圖：
示例：
①關(guān)于圖表的描述：
Relative abundance of XXX taxa (y axis as group percentage of the total number of XXX rRNA gene sequences per sample, after rarefaction to correct for uneven sampling effort) grouped by general XXX class. Identities of the sites sampled are given on the x axis.
②關(guān)于圖表結(jié)果的描述：
?The rarified sequence data set suggests that soils from our sites were overwhelmingly dominated by taxa in the XXX with the Alveolata and Cercozoa representing 66.5% and 22.5%[具體數(shù)據(jù)來源于物種分布表], respectively, of all of the protistan sequences recovered. The green algae (Archaeplastida, Chloroplastida, Chlorophyta) were also highly abundant in a few soils from XX areas, as were the golden algae (SAR, Stramenopiles, Chrysophyceae)[圖片展示直觀描述]. Our finding that Cercozoa (diverse flagellates and amoebae within Rhizaria) and Ciliophora (one of three major groups within Alveolata, along with dinoflagellates and Apicomplexa) are common members of eukaryotic soil microbial communities is congruent with what we know from previously published direct observation studies of soil protistan communities[reference][圖片展示直觀陳述，與前人工作結(jié)果一致性]. The only comparable study using high-throughput pyrosequencing to examine terrestrial protistan diversity[reference], also found alveolates and Rhizaria to be the dominant protists in soil [圖片展示的直觀陳述，本研究手段的發(fā)現(xiàn)].
b）優(yōu)勢(shì)物種（關(guān)鍵物種） Heatmap 圖：
示例：
①圖表描述：
Heat map of XXX key OTUs responding to XXX and XXX treatment identified by XXX models. The colour of the spots in the panel represents the mean relative abundance (normalized and log-transformed) of the OTU in each group. The values on the colour bar are the relative abundance indicated by the corresponding colour.
②關(guān)于圖表結(jié)果的描述：
?XXX models identified XX OTUs responding to XX treatment and XX OTUs responding to XX augmentation. In total, XX non-redundant OTUs contributed significantly to the establishment of the models. XX OTUs were significantly enriched by A treatment. These OTUs belonged to [物種名稱](差異分析結(jié)果). A total of XX OTUs were significantly decreased, most of which were species from the families [物種名稱]. ……
c）OTU 分布 Venn 圖
示例：
①圖表描述：
The Venn diagram depicts OTUs that were unique to the XX cows used in this study (COWS), unique to the public repositories (REF) or shared.
②關(guān)于圖表結(jié)果的描述：
Out of? XXX bacterial species-level OTUs found in this study, only XXX (XX%) were shared with the three public repositories. Moreover, for every OTU found in the public repositories, a novel one was discovered from the new data, while still far from maximal Good’s coverage. Conversely, the majority of the archaeal OTUs (XX%) found in this study were previously observed in the public databases with only 2 new OTUs added; 1 [物種] and 1 [物種]. For Fungi, only 1 OTU was shared between the public repositories and the animals investigated in this study, suggesting the scientific community is only beginning to realize the extent of fungal diversity of ruminants. It should be noted that, of the XXX ruminal eukaryotic OTUs in public repositories, roughly XX % were fungal, which further highlights the paucity of rumen fungal rRNA sequences in these databases. ……
溫馨提示：
一般地，一些物種、組別之間的顯著性差異分析結(jié)果，可以在顯著差異分析名稱的文件夾下找到。
