由于基因表達跟細胞表面蛋白的表達具有時間差，所以要根據經驗具體情況具體分析，內容僅供參考

??哺乳類動物B細胞的分化過程主要可分為前B細胞、不成熟B細胞、 成熟B細胞、活化B細胞和漿細胞五個階段。

??其中前B細胞和不成熟B細胞的分化是抗原非依賴的，其分化過程在骨髓中進行。抗原依賴階段是指成熟B細胞在抗原刺激后活化，并繼續分化為合成和分泌抗體的漿細胞，這個階段的分化主要是在外周免疫器官中進行的。

Progenitor B Cell

CD34
CD117/c-kit
Flt-3/Flk-2
IL-7R alpha/CD127
Neprilysin/CD10
Pax5/BSAP
Sca-1/Ly6

Mouse B1 or B2 Progenitor Cell

B220/CD45R
C1qR1/CD93
CD19

Pre-Pro B Cell

B220/CD45R
C1qR1/CD93
CD19
CD24
CD34
CD38
CD43
CD117/c-kit
CXCR4
Flt-3/Flk-2
IL-7R alpha/CD127
Neprilysin/CD10
Pax5/BSAP

Pro-B Cell

B220/CD45R
C1qR1/CD93
CD19
CD24
CD34
CD38
CD43
CD117/c-kit
CD20
IL-3R alpha
IL-7R alpha/CD127
Neprilysin/CD10
Pax5/BSAP

Pre-B Cell

B220/CD45R
C1qR1/CD93
CD19
CD24
CD34
CD38
CD43
CD117/c-kit
CD20
IL-3R alpha
IL-4R alpha
IL-7R alpha/CD127
Neprilysin/CD10
Pax5/BSAP

Immature B Cell

B220/CD45R
C1qR1/CD93
CD19
CD21
CD23/Fc epsilon RII
CD24
CD27/TNFRSF7
CD38
CD40/TNFRSF5
CD43
CD117/c-kit
CD20
IL-4R alpha
IL-7R alpha/CD127
Neprilysin/CD10

Human Transitional B Cell

C1qR1/CD93
CD5
CD19
CD21
CD23/Fc epsilon RII
CD24
CD27/TNFRSF7
CD38
Neprilysin/CD10
TACI/TNFRSF13B
BR3

Mouse Transitional 1 (T1) or Transitional 2 (T2) B Cell (from B2 Progenitor Cell)

B220/CD45R
C1qR1/CD93
CD19
CD24
CD43

Mouse B1a or B1b Cell (from B1 Progenitor Cell)

CD1d
CD5
CD19
CD23/Fc epsilon RII
CD43

Marginal Zone B Cell 邊緣區（MZ）B細胞

B220/CD45R
C1qR1/CD93
CD1c/BDCA-1
CD1d
CD19
CD21
CD23/Fc epsilon RII
CD27/TNFRSF7
CD43
CD20
FCRL3/FcRH3
TACI/TNFRSF13B

Follicular B Cell 濾泡（FO）B細胞

B220/CD45R
CD1d
CD19
CD21
CD23/Fc epsilon RII
CD24
CD27/TNFRSF7
CD38
CD43
CD20
CXCR5
MHC class II (I-A/I-E)
Neprilysin/CD10
Siglec-2/CD22
TACI/TNFRSF13B

Activated Germinal Center B Cell

B220/CD45R
CD19
CD27/TNFRSF7
CD38
CD40/TNFRSF5
CD83
CD20
MHC class II (I-A/I-E)
TACI/TNFRSF13B
BR3

Memory B Cell

B220/CD45R
C1qR1/CD93
CD19
CD21
CD27/TNFRSF7
CD40/TNFRSF5
CD20
MHC class II (I-A/I-E)
TACI/TNFRSF13B
CD80
CD84
CD86

Plasmablast

B220/CD45R
BCMA/TNFRSF17
C1qR1/CD93
CD19
CD27/TNFRSF7
CD38
Syndecan-1/CD138

Plasma Cell

B220/CD45R
BCMA/TNFRSF17
BLIMP1/PRDM1
CD19
CD27/TNFRSF7
CD38
CD20
CXCR4
MHC class II (I-A/I-E)
Syndecan-1/CD138
CD139
CD78

Regulatory B Cell 調節性B（Breg）細胞

B220/CD45R
B7-1/CD80
B7-2/CD86
BLIMP1/PRDM1
CD1d
CD5
CD11b/Integrin alpha M
CD19
CD21
CD23/Fc epsilon RII
CD24
CD27/TNFRSF7
CD38
CD40/TNFRSF5
CD44
CD69
CD72
CD20
CD25/IL-2R alpha
CXCR4
DEP-1/CD148
EBI3
EMMPRIN/CD147
HLA-DR
IgM
IL-10
IL-12 R beta 2
IL-12/IL-35 p35
IL-21
IL-21R
IL-23/IL-39 p19
IL-27 R alpha/WSX-1/TCCR
IL-39 (IL-23p19/EBI3)
IRF4
Siglec-2/CD22
STAT1
STAT3
Syndecan-1/CD138
TACI/TNFRSF13B
TGF-beta
TIM-1/KIM-1/HAVCR
TLR4

未成熟B細胞的Marker

• 未成熟的B細胞表達CD19，CD 20，CD34，CD38和CD45R，但不表達IgM。

成熟B細胞的Marker

• 對于大多數成熟的B細胞，關鍵Markers包括IgM和CD19（一種抗原蛋白受體）
  • 活化的B細胞：CD30+（一種凋亡調節劑）
  • 血漿B細胞：CD19-CD78+

一個Marker基因查找數據庫

http://biocc.hrbmu.edu.cn/CellMarker/

參考文章

• https://www.labome.com/method/T-Cell-Markers-and-B-Cell-Markers.html
• https://www.rndsystems.com/cn/research-area/b-cell-markers
• https://www.abcam.com/primary-antibodies/b-cells-basic-immunophenotyping
• https://ashpublications.org/blood/article/128/22/2108/100501/B-Cell-Markers-Predict-Response-to-Venetoclax-in