下載aspera
軟件官網：asperasoft.com
wget -c https://download.asperasoft.com/download/sw/connect/3.8.1/ibm-aspera-connect-3.8.1.161274-linux-g2.12-64.tar.gz
tar -zxvf ibm-aspera-connect-3.8.1.161274-linux-g2.12-64.tar.gz
sh aspera-connect-3.7.4.147727-linux-64.sh
這一步推薦下載3.9.0以前的版本，我下載了最新的版本沒有找到私鑰，下載文件過程中會報錯。
查看軟件
1.軟件下載至home目錄下，進入home目錄查看
cd #
ls -a
tree 3 L .aspera/ #樹形展示.aspera/文件下3級目錄
2.批量下載數據
for i in {9..16}
do
a0='~/.aspera/connect/bin/'
a1='ascp -QT -l 300m -P33001 -i ~/.aspera/connect/etc/asperaweb_id_dsa.openssh era-fasp@fasp.sra.ebi.ac.uk:/vol1/fastq/ERR331/00'
a2=$(($i % 10))
a3='/ERR33103'$i
a4='_1.fastq.gz .'
a5='_2.fastq.gz .'
echo $a0$a1$a2$a3$a3$a4
echo $a0$a1$a2$a3$a3$a5
done >>ascp.command
nohup bash ascp.command & #后臺下載，如果不是很熟練的話，建議前臺下載。
下面上以上循環的網站，大家可以根據自己所下載文件的編號進行修改
ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/009/SRR1805929/SRR1805929_1.fastq.gz
ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/009/SRR1805929/SRR1805929_2.fastq.gz
ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/000/SRR1805930/SRR1805930_1.fastq.gz
ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/000/SRR1805930/SRR1805930_2.fastq.gz
ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/001/SRR1805931/SRR1805931_1.fastq.gz
ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/007/SRR1805937/SRR1805937_1.fastq.gz
ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR180/001/SRR1805951/SRR1805951_1.fastq.gz
3.下載
正常的話現在就可以下載了
Session Stop (Error: Server aborted session: No such file or directory) #這個就是個位數所導致的出錯，后面又單獨下載了。
Session Stop (Error: Server aborted session: No such file or directory)
ERR3310310_1.fastq.gz 100% 1512MB 289Mb/s 00:54
Completed: 1548504K bytes transferred in 55 seconds
(229439K bits/sec), in 1 file.
ERR3310310_2.fastq.gz 100% 1773MB 290Mb/s 00:53
Completed: 1816554K bytes transferred in 54 seconds
(274884K bits/sec), in 1 file.
ERR3310311_1.fastq.gz 100% 1695MB 288Mb/s 00:56
Completed: 1736188K bytes transferred in 57 seconds
(245472K bits/sec), in 1 file.
4.下載完成后即可進行后續質控分析了
