文獻DOI：10.1007/s40618-017-0720-0
文獻PMID：28660607
文獻原文鏈接：http://doi.org.sci-hub.tw/10.1007/s40618-017-0720-0
The effects of alpha?lipoic acid on diabetic myopathy
α-硫辛酸對糖尿病肌病的影響
【Abstract】
Purpose： Increased oxidative stress and impaired antioxidant defense are important mechanisms in the pathogenesis of diabetic myopathy. Since diabetes mellitus type 1 decreases muscle regeneration capacity the present study was designed to determine the infuence of alpha-lipoic acid (ALA), a potent biological antioxidant, on the process of regeneration of diabetic rat skeletal muscles.
Methods： 40 Wistar rats were divided into three groups: control (n = 8), untreated diabetic group (n = 16) and ALA treated diabetic group (n = 16). The regeneration process was provoked in streptozotocin-induced diabetic rats in both slow (m.soleus, SOL) and fast (m.extensor digitorum longus, EDL) skeletal muscles by intramuscular injection of myotoxin bupivacaine. At intervals of 10 days and 4 weeks, muscle histochemical and morphometrical analysis (fber cross areas and fber type distribution) was performed.
Results： Changes induced by diabetes are evident in redistribution of muscle fbers and in signifcant level of atrophy. After 4 weeks of diabetes, glycolytic muscle fbers are dominant in both slow and fast muscles. Muscle atrophy is present in all fber types except in type I of slow skeletal muscle. Treatment with ALA reduce changes in the morphological properties caused by diabetes mellitus type 1 in slow and fast rat skeletal muscles during the process of regeneration.
Conclusion：Treatment with lipoic acid during 4 weeks has shown effects on the redistribution of muscle fbers, and can prevent atrophy in slow and fast diabetic muscle.
Keywords： Skeletal muscle · Regeneration · Diabetes mellitus type 1 · Alpha-lipoic acid
摘要翻譯：
目的：增加氧化應激和抗氧化防御受損是糖尿病肌病發病機制的重要機制。由于1型糖尿病降低了肌肉再生能力，本研究旨在確定α-硫辛酸（ALA）（一種有效的生物抗氧化劑）對糖尿病大鼠骨骼肌再生過程的影響。
方法：將40只Wistar大鼠分為3組：對照組（n = 8），未治療的糖尿病組（n = 16）和ALA治療的糖尿病組（n = 16）。通過肌內注射肌肉毒素布比卡因，在慢性（m.soleus，SOL）和快速（m.extensor digitorum longus，EDL）骨骼肌中的鏈脲佐菌素誘導的糖尿病大鼠中引起再生過程。以10天和4周的間隔，進行肌肉組織化學和形態計量學分析（纖維交叉區域和纖維類型分布）。
結果：糖尿病引起的變化在肌肉纖維重新分布和顯著萎縮水平中是明顯的。糖尿病4周后，慢速和快速肌肉的肌纖維都是以糖酵解占主導地位。除了緩慢骨骼肌的I型外，所有肌肉類型都存在肌肉萎縮。 ALA治療減少了在慢速和快速大鼠骨骼肌中再生過程中由1型糖尿病引起的形態學特性的變化。
結論：4周內用硫辛酸治療對肌肉纖維的再分布有一定的作用，可以預防慢性和快速糖尿病肌肉的萎縮。
關鍵詞：骨骼肌·再生·糖尿病1型·α-硫辛酸
