fastq文件詳解

@EAS139:136:FC706VJ:2:2104:15343:197393 1:Y:18:ATCACG

EAS139the unique instrument name

136the run id

FC706VJthe flowcell id

2flowcell lane

2104tile number within the flowcell lane

15343‘x’-coordinate of the cluster within the tile

197393‘y’-coordinate of the cluster within the tile

1the member of a pair, 1 or 2(paired-end or mate-pair reads only)

YY if the read fails filter (read is bad), N otherwise

180 when none of the control bits are on, otherwise it is an even number

ATCACGindex sequence

更詳細的信息見 ?fastq(薄耘生物)

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