• Cladosporols A 和 B 是兩種天然過氧化物酶體增殖物激活受體γ（PPARγ）激動(dòng)劑，可抑制 3 T3-L1 前脂肪細(xì)胞的脂肪形成，并導(dǎo)致 HT-29 細(xì)胞增殖的條件培養(yǎng)基依賴性停滯- ScienceDirect --- Cladosporols A and B, two natural peroxisome proliferator-activated receptor gamma (PPARγ) agonists, inhibit adipogenesis in 3T3-L1 preadipocytes and cause a conditioned-culture-medium-dependent arrest of HT-29 cell proliferation - ScienceDirect

As expected, expression of the two early adipogenesis-related genes, PPARγ and C/EBPα, was significantly reduced after treatment with MDI plus 5 μM cladosporol A or cladosporol B for 3 days (Fig. 3B). Furthermore, the mRNA level of four of terminal differentiation markers (aP2, FASN, adiponectin and perilipin) was diminished when the cells were treated with MDI plus 5 μM cladosporol A or cladosporol B for 10 days, compared with cells treated with MDI or MDI plus RGZ (Fig. 3C).

兩種早期脂肪生成相關(guān)基因 PPARγ和 C/EBPα的表達(dá)顯著降低（ 圖 3B）。此外，與用 MDI 或 MDI 加 RGZ 處理的細(xì)胞相比，當(dāng)用 MDI 加 5μM 枝孢酚 A 或枝孢酚 B 處理細(xì)胞 10 天時(shí)，四種終末分化標(biāo)志物（aP 2、FXR、脂聯(lián)素和圍脂蛋白）的 mRNA 水平降低