一、 DAP-seq技術簡介

DAP-seq（DNA親和純化測序）技術服務——高通量檢測轉錄因子或DNA結合蛋白在基因組上的結合位點。

在功能基因組學和表觀遺傳學研究中，轉錄因子結合位點（TFBS）的發掘一直是研究熱點。傳統的ChIP-seq（染色質免疫共沉淀測序）方法，在抗體質量很好的情況下能夠有效檢測到TFBS。然而，好的抗體可遇不可求，這限制了ChIP-seq更廣泛的應用。

2016年，O'Malley RC等人在Cell上發表了使用DAP-seq技術，快速繪制轉錄因子調控靶向DNA區域圖譜的文章。2017年，Bartlett A等人在Nature Protocol上發表了 DAP-seq的實驗方法。 DAP-seq技術的出現，使TFBS 的研究不再局限于物種，不再受抗體質量的限制，為生命科學和醫學領域轉錄因子的研究提供了新的有效工具。

二、 ChIP-seq和DAP-seq技術對比

三、DAP-seq技術流程：

藍景科信已發表文章列表：

Zhang SL, Wang L, Yao J, Wu N, Ahmad B, Nocker S, Wu JY, Abudureheman R, Li Z, Wang XP. Control of ovule development in Vitis vinifera by VvMADS28 and interacting genes. Horticulture Research. 2023. doi: 10.1093/hr/uhad070.?(IF=7.291)?

Wang L, Tian T, Liang J, Li R, Xin X, Qi Y, Zhou Y, Fan Q, Ning G, Becana M, Duanmu D. A transcription factor of the NAC family regulates nitrate-induced legume nodule senescence. New Phytol. 2023 Mar 22. doi: 10.1111/nph.18896. (IF=10.323)

Sun Y, Han Y, Sheng K, Yang P, Cao Y, Li H, Zhu QH, Chen J, Zhu S, Zhao T. Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of pigment glands in Gossypium bickii. Mol Plant. 2023. doi: 10.1016/j.molp.2023.02.005. (IF=21.949)?

Liu Y, Liu Q, Li X, Zhang Z, Ai S, Liu C, Ma F, Li C. MdERF114 enhances the resistance of apple roots to Fusarium solani by regulating the transcription of MdPRX63. Plant Physiol. 2023. doi: 10.1093/plphys/kiad057. (IF=8.005)?

Liu YN, Wu FY, Tian RY, Shi YX, Xu ZQ, Liu JY, Huang J, Xue FF, Liu BY, Liu GQ. The bHLH-zip transcription factor SREBP regulates triterpenoid and lipid metabolisms in the medicinal fungus Ganoderma lingzhi. Commun Biol. 2023. doi: 10.1038/s42003-022-04154-6. (IF=6.548)

Liu L, Chen G, Li S, Gu Y, Lu L, Qanmber G, Mendu V, Liu Z, Li F, Yang Z. A brassinosteroid transcriptional regulatory network participates in regulating fiber elongation in cotton. Plant Physiol. 2022. doi: 10.1093/plphys/kiac590. (IF=8.005)

Li M, Hou L, Zhang C, Yang W, Liu X, Zhao H, Pang X, Li Y. Genome-Wide Identification of Direct Targets of ZjVND7 Reveals the Putative Roles of Whole-Genome Duplication in Sour Jujube in Regulating Xylem Vessel Differentiation and Drought Tolerance. Front Plant Sci. 2022 Feb 4;13:829765. doi: 10.3389/fpls.2022.829765. (IF=6.627)

Bi Y, Wang H, Yuan X, Yan Y, Li D, Song F. The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6. J Integr Plant Biol. 2022. doi: 10.1111/jipb.13399. (IF=9.106)

Guo X, Yu X, Xu Z, Zhao P, Zou L, Li W, Geng M, Zhang P, Peng M, Ruan M. CC-type glutaredoxin, MeGRXC3, associates with catalases and negatively regulates drought tolerance in cassava (Manihot esculenta Crantz). Plant Biotechnol J. 2022. doi: 10.1111/pbi.13920. (IF=13.263)

Chai Z, Fang J, Huang C, Huang R, Tan X, Chen B, Yao W, Zhang M. A novel transcription factor, ScAIL1, modulates plant defense responses by targeting DELLA and regulating gibberellin and jasmonic acid signaling in sugarcane. J Exp Bot. 2022. 73: 6727-6743. doi: 10.1093/jxb/erac339. (IF=7.298)

Li R, Zheng W, Yang R, Hu Q, Ma L, Zhang H. OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice. Plant J. 2022. 112: 151-171. doi: 10.1111/tpj.15937. (IF=5.726)

Li Q, Zhou L, Chen Y, Xiao N, Zhang D, Zhang M, Wang W, Zhang C, Zhang A, Li H, Chen J, Gao Y. Phytochrome interacting factor regulates stomatal aperture by coordinating red light and abscisic acid. Plant Cell. 2022. 34: 4293-4312. doi: 10.1093/plcell/koac244. (IF=12.085)

Luo M, Lu B, Shi Y, Zhao Y, Wei Z, Zhang C, Wang Y, Liu H, Shi Y, Yang J, Song W, Lu X, Fan Y, Xu L, Wang R, Zhao J. A newly characterized allele of ZmR1 increases anthocyanin content in whole maize plant and the regulation mechanism of different ZmR1 alleles. Theor Appl Genet. 2022. 135: 3039-3055. doi: 10.1007/s00122-022-04166-0. (IF=5.574)

Wei H, Xu H, Su C, Wang X, Wang L. Rice CIRCADIAN CLOCK ASSOCIATED 1 transcriptionally regulates ABA signaling to confer multiple abiotic stress tolerance. Plant Physiol. 2022. 190: 1057-1073. doi: 10.1093/plphys/kiac196. (IF=8.005)

Tang N, Cao Z, Yang C, Ran D, Wu P, Gao H, He N, Liu G, Chen Z. A R2R3-MYB transcriptional activator LmMYB15 regulates chlorogenic acid biosynthesis and phenylpropanoid metabolism in Lonicera macranthoides. Plant Sci. 2021. 308: 110924. doi: 10.1016/j.plantsci.2021.110924. (IF=5.363)

Liang S, Gao X, Wang Y, Zhang H, Yin K, Chen S, Zhang M, Zhao R. Phytochrome-interacting factors regulate seedling growth through ABA signaling. Biochem Biophys Res Commun. 2020. 526: 1100-1105. doi: 10.1016/j.bbrc.2020.04.011. (IF=3.322)

Yao J, Shen Z, Zhang Y, Wu X, Wang J, Sa G, Zhang Y, Zhang H, Deng C, Liu J, Hou S, Zhang Y, Zhang Y, Zhao N, Deng S, Lin S, Zhao R, Chen S. Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance. J Exp Bot. 2020. 71: 1527-1539. doi: 10.1093/jxb/erz493. (IF=5.36)

四、DAP-seq實驗流程

五、DAP-seq生信分析內容

1.對原始數據進行去除接頭、污染序列及低質量 reads 的處理

2.數據產出統計

3.參考序列比對分析?

4.測序reads富集區域掃描（peak calling）

5.Peak長度分布統計

6.Peak在基因功能元件上的分布統計

7.Peak序列模式發掘（motif search）

8.已知motif注釋

9.Peak相關基因鑒定

10.Peak相關基因的GO和KEGG富集分析

11.測序數據的差異分析（>=2個樣本）

12.測序數據的可視化分析